Washington University in St. Louis

The Patti Lab
Metabolomics to elucidate novel biochemical mechanisms of disease
Job Opportunities
Job opportunities are available.

Differential Incorporation of Glucose into Biomass during Warburg Metabolism

Chen Y-J, Huang X, Mahieu NG, Cho K, Schaefer J, Patti GJ
Differential Incorporation of Glucose into Biomass during Warburg Metabolism
Biochemistry, 53(29), 4755-4757, 2014
doi:10.1021/bi500763u

It is well established that most cancer cells take up an increased amount of glucose relative to that taken up by normal differentiated cells. The majority of this glucose carbon is secreted from the cell as lactate. The fate of the remaining glucose carbon, however, has not been well-characterized. Here we apply a novel combination of metabolomic technologies to track uniformly labeled glucose in HeLa cancer cells. We provide a list of specific intracellular metabolites that become enriched after being labeled for 48 h and quantitate the fraction of consumed glucose that ends up in proteins, peptides, sugars/glycerol, and lipids.

Washington University, Departments of Chemistry, Genetics, and Medicine. Saint Louis, Missouri 63110 USA